Composite

Part:BBa_K569006:Design

Designed by: David Golynskiy and Tyler Guinn   Group: iGEM11_UT_Dallas   (2011-09-27)

ctx+CheY


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 428
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal PstI site found at 428
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 428
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 428
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We designed and used primers to PCR out both the CTX from CTX-GFP and Chey from an old backbone 

       CTX
            foward-5'-GTA TCA CGA GGC AGA ATT TCA -3'
            reverse-5'-CCAactagtTTTCTCCTCTTTAATCTCTAGTACAGAA-3'        
       CheY 
            foward-5'-ccatctagacacctgcaaaaggagatgg-3'
            revers-5'-ccaactagtcacctgcaaaaagggacat-3'

Source

J07011 (MIT)

K422007 (Basel)

References